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Different polypeptide solubility, which consists of peptide sequence is determined by amino acid solubility. In general to take a small amount of peptides with deionized water or distilled water dissolving experiment, when the peptide completely dissolved after adding water or dilute buffer to the final concentration. If polypeptide dissolved in deionized water in the solution is not good, can add just the right amount of acetonitrile help dissolve, ultrasonic helps dissolve polypeptide. In the case of adding acetonitrile peptide was still in bad solubility, consider changing the PH of the solution to help dissolve. Peptide sequence contains basic amino acid, the more add a small amount of dilute acetic acid solution (10%), help to dissolve. Peptide sequence of acidic amino acid, the more add a moderate amount of ammonia solution helps to dissolve. For more difficult soluble polypeptide contains hydrophobic amino acids, can use a small amount of solvent DMF and DMSO help dissolve, suggested that dissolved the peptides in the high concentration of solvent and then adding water or buffer concentration of diluted to normal use.
Customers receive peptide product in general is a solid powder. Peptide absorption of moisture reduces the stability, so customers pay attention to in the process of using dry preservation, the use of desiccant. In addition in the preservation process put into the freezer - 20 ℃ save, and avoid too large fluctuations in temperature to maintain the stability of polypeptide. If kept in a liquid, PH value had better adjust to 5-6, use sterilized buffer, - 20 ℃ in order to prolong the service life of peptides. Peptides in transit temperature change will not affect the quality of product.