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Select the appropriate fluorescent tags group depends on your experimental requirements. Pharmacology experimental study basic divided into two categories: in vivo and in vitro study. For in vivo studies, generally choose to launch in 650-900 - nm wavelength fluorescent groups, such as: ICG and Nile Blue. This is because the fluorescence emitted from such groups has good tissue penetration, the background interference is smaller. For in vitro study, 400 to 600 nm fluorescence emission wavelength in groups is most commonly used, for example: AMC, FITC and TAMRA.
Active peptide can be fluorescent groups, fluorescence imaging applications. Fluorescent groups in molecule has the following functions: the absorb certain wavelengths after the photon energy, immediately in a relatively long wavelength region releases a certain energy photons. For fluorescent groups dyeing ability and efficiency, a series of reference standards. In these standards, molar extinction coefficient QY epsilon and quantum efficiency are the two most important to measure the parameters of the fluorescence intensity, which is closely related to the photon absorption and fluorescence emission.