E.Coli Expression Services

E. coli expression system is the development of the earliest in the field of gene expression and currently the most widely has been used classic expression system. Compared with the other expression systems, e. coli expression system has a clear genetic background, the purpose gene expression level is high, short cultivation period, strong ability to resist pollution characteristics, occupies an important position in the technology of gene expression, is an important tool in the development process of molecular biology and biotechnology industrialization.

Service process

1, the whole gene synthesis of the target gene

2, the target gene cloning

3, expression strain transformation and filtering

4, the target protein expression and purification

The project content

1, the whole gene synthesis:

 contains the purpose gene synthesis and cloning, optional expression vector is given priority with PET and pGEX series.

2, the original nucleoprotein expression and purification:

including the E.coli expression strain transformation, filtering , expression and purification;

optional expression of strainl : BL21 (DE3), Rosetta - gami (DE3), OrigamiB (DE3), Rosetta (DE3).

Product delivery

the finished product in the each stage of service process, including:

purpose gene (conventional shuttle carrier or in T carrier);

the recombinant expression plasmid of right construct;

including the expression strains of recombinant plasmid ;

target protein after purification