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Mammalian cell expression system Service process 1, The whole gene synthesis of target gene 2, the target gene cloning to mammalian cell expression vector 3, small transfection and finish Western Blot test 4, the target protein expression and purification 5, The target protein detection 6, Screening of high stable expression cell lines
The project content 1, the whole gene synthesis:contains the purpose gene synthesis and the cloning, optional expression vector, for example, PcDNA3.1 eukaryotic expression vector 2, mammalian cell expression and purification: including small transfection and finish Western Blot test, target protein expression and purification; Screening of high stable expression cell lines (Lengthen the service cycle); the optional expression cell line ; 293, 293 t, etc.
Product delivery the finished product in the each stage of service process, including: the correct build eukaryotic expression plasmid; target protein after purification. According to customer's request, provide the finished protein, which contains purified tag or removed purification label tag, up to more than 90% purity, and complete service report. |